Mapping the MHC class I spliced immunopeptidome of cancer cells
Description
Fig. 1 (+ 2D GR-LCL) files: filteredSearchResults.xlsx. Listed are all peptides identified in the HCT116, HCC1143 and 2D GR-LCL MHC-I immunopeptidomes (spliced and non-spliced, 9-12mer sequences) with MS/MS scan numbers, retention times, mass characteristics and ion scores. Fig. 1C-E File: LyS-tryp_HCC1143_30KDa.RAW LysC/trypsin digestion of the HCC1143 protein sample (proteins > 30kDa) measured by Q Exactive Orbitrap Fig. 3C Folder: HCC1143_mutation_cosmic_database Mutations of the HCC1143 cell line as reported in the Cosmic database (version 17/8/2016) files: Mutations_in_sampleWed Aug 17 16-37-54 2016.csv Mutations_in_sampleWed Aug 17 16-38-01 2016.csv Mutations_in_sampleWed Aug 17 16-38-09 2016.csv Mutations_in_sampleWed Aug 17 16-38-18 2016.csv Fig. 3C-E Folder: HCT116_mutation_cosmic_database Mutations of the HCT116 cell line as reported in the Cosmic database (version 17/8/2016) files: Mutations_in_sampleWed Aug 17 17-09-53 2016.csv Fig. S5 Folder: in_vitro_digestions files: RAW files of the in vitro digestions (with or without proteasome) of the synthetic polypeptides mutCHMP7 and mutRBBP7 (see Table S6) and the target synthetic peptides’ run. The MS/MS of the identified neoepitopes are shown in Fig. S5. In particular: - “synthetic_peptides.RAW” is a mix containing also the synthetic peptides CHMP7[A324T]_316-325 and RBBP7[N17D]_12-20 - “mutCHMP7_20h.RAW” is the digestion (20h) of the synthetic substrate mutCHMP7_312-330 with purified proteasome - “mutCHMP7_20h_no-proteasome.RAW” is the digestion (20h) of the synthetic substrate mutCHMP7_312-330 without purified proteasome - “mutRBBP7_20h.RAW” is the digestion (20h) of the synthetic substrate mutRBBP7_6-25 with purified proteasome - “mutRBBP7_20h_no-proteasome.RAW” is the digestion (20h) of the synthetic substrate mutRBBP7_6-25 without purified proteasome The scripts for the MHC-I spliced peptides’ database generation Folder: TourDeForce
Files
Steps to reproduce
see Material and Method section in the main text and Supplemental Experimental Procedure in the Supplementary Material