Large DNA Methylation Nadirs Anchor Chromatin Loops Maintaining Hematopoietic Stem Cell Identity

Published: 10 Feb 2020 | Version 1 | DOI: 10.17632/y56xsrrrp8.1
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Description of this data

-The co-immunostaining of NUP98 and H3K27me3 in CD34+ cells treated with DMSO and EPZ6438 (0.5μM). H3K27me3 is stained with Alexa 594 secondary antibody. After EPZ6438 (0.5μM), we can see a significant reduction in the H3K27me3 levels.
-FISH imaging of canyon loci in CD34+ cells

Experiment data files

  • CD34 immunostaining
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  • FISH in CD34+ HSPC
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Latest version

  • Version 1

    2020-02-10

    Published: 2020-02-10

    DOI: 10.17632/y56xsrrrp8.1

    Cite this dataset

    Zhang, Xiaotian; Jeong, Mira; Liu, Yushuai (2020), “Large DNA Methylation Nadirs Anchor Chromatin Loops Maintaining Hematopoietic Stem Cell Identity”, Mendeley Data, v1 http://dx.doi.org/10.17632/y56xsrrrp8.1

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Institutions

Van Andel Institute, Baylor College of Medicine

Categories

Fluorescence in Situ Hybridization, Fluorescence Imaging, Hematopoietic Stem Cell

Licence

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