Human innate immune cytokine response to dengue virus
Published: 3 December 2020| Version 1 | DOI: 10.17632/y59s4xhpzn.1
Fluorescence minus four controls for flow cytometry experiments of innate immune cytokine production in response to dengue virus. Pseudo color flow cytometry plots for a representative patient comparing the fluorescence minus four (FMF; top row) control with completely stained sample (bottom) for secretion of (A) TNF-α, (B) IP-10, (C) IL-10, (D) IL-6 and (E) IFN-γ from the indicated cell subsets. Abbreviations: CM – CD14+CD16- classical monocytes; IM – CD14+CD16+ intermediate monocytes; NCM – CD14-CD16+ non-classical monocytes; G – Granulocytes; NKT – CD56+CD3+ natural killer T cells; NK++ - CD56+CD16+ natural killer cells; NK+- - CD56+CD16- natural killer cells; B – CD19+ cells.
Indian Institute of Science Department of Microbiology and Cell Biology
Immunology, Flow Cytometry, Innate Immunity, Dengue Fever