Lipidomics dataset of PTEN deletion-induced optic nerve regeneration mouse model

Published: 30-10-2020| Version 1 | DOI: 10.17632/y5h8vk3xmw.1
Contributor:
Sanjoy Bhattacharya

Description

Adult PTENloxP/loxP mice subjected to intravitreal injection of adeno-associated viruses expressing Cre (AAV-Cre) as a model of regeneration. At 4-week-old, loxP mice were intravitreally-injected with 2-3 μl of either AAV-Cre(KO) or AAV-PLAP (control), and two weeks later optic nerve crush was performed. At indicated time-points after crush (0 days, 7 days, 14 days), mice were euthanized and optic nerves were immediately dissected out, and then flash frozen on dry ice. The Bligh and Dyer method was used for lipid extraction, followed by mass spectrometry lipid profiling with a Q-Exactive Orbitrap Liquid Chromatography-Mass Spectrometer (LC MS-MS). The raw scans were analyzed with LipidSearch 4.2 and the statistical analysis was conducted through Metaboanalyst 4.0.

Files