Johannsen et al., Modification of VAMS, 2024

Published: 4 April 2024| Version 1 | DOI: 10.17632/y5jzfchd6f.1
Christina Johannsen,


Revolutionizing biomarker analysis one tip at a time – binding antibody to Volumetric Absorptive Microsampling tips for sensitive LC-MS analysis Johannsen C, Reubsaet L & Halvorsen TG Section of Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, Oslo, Norway Highlights • Novel antibody binding to VAMS tips enables instant immunocapture (‘Affinity-VAMS’) • Affinity-VAMS show promising efficacy in sample cleanup for targeted LC-MS analysis • Streamlined optimization significantly reduced production time of Affinity-VAMS • The target analyte was quantified in human serum samples using Affinity-VAMS Abstract The constant pursuit of efficient and cost-effective methods for analyzing human samples drives ongoing innovation in biomedical research. Introduced in 2014, Volumetric Absorptive Microsampling (VAMS) technology ensures fixed blood volume absorption and enhanced sample uniformity. Our study aims to enhance VAMS functionality by integrating sample preparation onto the device primarily used for sample collection. This involves the adsorption of antibodies onto VAMS tips, enabling instant sample clean up at the time of sample collection. Using human chorionic gonadotropin (hCG) as a model analyte, we evaluated the qualitative and quantitative performance of Affinity-VAMS. First, we showed that adsorbing and covalently binding monoclonal antibodies to VAMS tips results in binding of the target analyte in comparison to unmodified VAMS. Due to the ease of preparation, we moved forward using antibody adsorption to VAMS tip and optimized the procedure. Optimization of the procedure involved fine-tuning the antibody binding step, washing process, and determining the optimal amount of antibody required, leading to a streamlined process with significant time savings up to two days. Recovery experiments demonstrate successful capture of the target analyte by the Affinity-VAMS, while matrix effects and stability assessments indicate no negative effects from serum matrix or storage conditions. Finally, quantitative analysis shows promising performance of the Affinity-VAMS in detecting different concentrations of the target analyte in the concentration range between 2.5 - 25 ng·mL-1. The calculated correlation factor was R2 of 0.9987 and limit of detection 2.5 ng·mL-1. Calculations of accuracy and precision lay within the ICH guidelines for bioanalytical samples. While this report is focused on the proof of principle of the Affinity-VAMS, our findings demonstrate the potential for the usage of modified VAMS in remote sampling and integrated sample processing, enhancing biomarker analysis in clinical and research settings.


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Raw Data from targeted LC-MS/MS analysis


Universitetet i Oslo


Analytical Chemistry, Mass Spectrometry, Protein, Bioanalysis, Liquid Chromatography Tandem Mass Spectrometry