Rawa data accreta

Published: 06-07-2021| Version 1 | DOI: 10.17632/y6936wrfty.1
Contributor:
Berry Bancin

Description

Raw data of sFlt-1 and PLGF

Files

Steps to reproduce

Assay Procedure 1) Prepare all reagents before starting assay procedure. It is recommended that all Standards and samples be added in duplicate to the Microelisa Stripplate. 2) Set standard wells and testing sample wells, add standard 50μl to each standard well. add sample 50μl to each testing sample well 3) Add 100μl of HRP-conjugate reagent to each well except the blank well, cover with an adhesive strip and incubate for 60 minutes at 37°C 4) Wash the Microtiter Plate 4 times. Manual Washing - Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with Wash Solution (1×), then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure for a total of four times. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate frame firmly when washing the plate to assure that all strips remain securely in frame. Automated Washing - Aspirate all wells, then wash plates four times using Wash Buffer (1×). Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350μl/well/wash. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. 5) Add Chromogen Solution A 50μl and Chromogen Solution B 50μl to each well successively. Gently mix and then protect from light to incubate for 15 minutes at 37°C. 6) Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing. 7) Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.