Decsi et al., 2025_DIB_article

Description

Plant leaf samples were collected from untreated and foliar-treated wheat stands with seed priming and seed priming inder drought stressed circumstances, and raw RNA-seq data were obtained from these samples using the Illumina-NGS method.

Steps to reproduce

The mRNA was purified from the good-quality wheat leaf samples with paramagnetic NEXTFLEX® Poly(A) Beads 2.0. After fragmentation, strand-specific NGS library preparation was performed using the NEXTFLEX® Rapid Directional RNA-Seq 2.0 kit. The resulting pooled libraries were sequenced on the Illumina NovaSeq 6000 next-generation genome sequencing platform using a deep sequencing technique (2x150 bp PE reads; ave-rage paired-end read count 50 million/sample). The quality-checked reads were then concatenated into a de novo transcriptome. This operation was performed using the available Trinity software (http://TrinityRNASeq.sourceforge.net). To perform differential expression analysis, the individual expression levels of the de novo transcriptome contigs must be assessed. Without a reference genome, the software generates a map of the transcripts. After mapping, the software quantifies the reads by considering the gene coordinates. The software records the summary of the results in a count table output file.

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Funders

• Magyar Agrár- és Élettudományi Egyetem

  Hungary

  Grant ID: Magyar Agrár- és Élettudományi Egyetem Research Excellence Programme of the Hungarian University of Agriculture and Life Sciences and Flagship Research Groups Programme of the Hungarian University of Agriculture and Life Sciences

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