16s rRna data of Bacterial community during the secondary composting of Black soldier fly larvae bio-convertion residue

Published: 7 April 2021| Version 2 | DOI: 10.17632/y7rzrbt6y5.2
Contributor:
Jingjin Ma

Description

In this study, the black soldier fly larvae bio-convertion technology was used for the treatment of food waste, and the diversity of bacterial community during the secondary composting process of the residual meterials of BSFL bio-convertion. The DNA was extracted from the residue by Fast soil DNA kit. Agarose gel electrophoresis was used to detect the integrity of the fragments. PCR amplification of the intact fragments was performed with positive and negative primers of 341F/806R, and the amplification region was in the V3-V4 region of 16S rRNA.Single-end sequencing was performed using the ION S5 XL high-throughput sequencing platform. These data are the original data obtained by sequencing. Samples are arranged in a time series with a sampling interval of 7 days, which can be analyzed after processing.

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Institutions

Zhejiang University

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Environment and Health

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