Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota

Published: 23 October 2019| Version 2 | DOI: 10.17632/y8c7vpc8zp.2
Contributors:
Timothy Jenkins,
Laura Peachey,
Nadim Ajami,
Andrew MacDonald,
Michael Hsieh,
Paul Brindley,
Cinzia Cantacessi,
Gabriel Rinaldi

Description

Raw 16S rRNA sequencing data from luminal contents from sections of the small (SI) and large intestines (LI) of 10 Swiss-Webster female mice (S+) that were infected with 200 S. mansoni cercariae and 10 age- and gender-matched mice that remained uninfected and were included as controls (S-). For each S+ and S- group, five mice were euthanised 28 days after infection (D28 p.i., i.e. before egg laying by sexually mature S. mansoni females had commenced). The remaining mice (n = 5 for each S+ and S-) were euthanised at day 50 p.i. (D50 p.i., corresponding with ongoing egg laying by sexually mature S. mansoni females). The experiment was carried out in duplicate and samples from both experiments (i.e. Exp. 1 & Exp. 2) pooled for further analysis. The data set includes four types of files: 1. Raw joined read files - 1 per sample (demultiplexed): e.g. Exp1_SI_S-_D50_1.fasta 2. Joined, quality filtered file containing reads from all samples and ready for OTU picking. The file was generated using Qiime: seqs.fna 3. Metadata file containing the relevant contextual information for each sample: Schisto_microbiome_Metadata.csv 4. OTU table created using Qiime including all of the taxonomic information: otu_table_mc2_w_tax_no_pynast_failures.biom

Files

Steps to reproduce

See publication

Institutions

Wellcome Sanger Institute, Manchester University, George Washington University School of Medicine and Health Sciences, University of Cambridge, Baylor College of Medicine

Categories

Microbiome, Mouse, Schistosoma, Schistosomiasis

License