Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota

Published: 23 Oct 2019 | Version 2 | DOI: 10.17632/y8c7vpc8zp.2
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Description of this data

Raw 16S rRNA sequencing data from luminal contents from sections of the small (SI) and large intestines (LI) of 10 Swiss-Webster female mice (S+) that were infected with 200 S. mansoni cercariae and 10 age- and gender-matched mice that remained uninfected and were included as controls (S-). For each S+ and S- group, five mice were euthanised 28 days after infection (D28 p.i., i.e. before egg laying by sexually mature S. mansoni females had commenced). The remaining mice (n = 5 for each S+ and S-) were euthanised at day 50 p.i. (D50 p.i., corresponding with ongoing egg laying by sexually mature S. mansoni females). The experiment was carried out in duplicate and samples from both experiments (i.e. Exp. 1 & Exp. 2) pooled for further analysis.

The data set includes four types of files:

  1. Raw joined read files - 1 per sample (demultiplexed): e.g. Exp1_SI_S-_D50_1.fasta

  2. Joined, quality filtered file containing reads from all samples and ready for OTU picking. The file was generated using Qiime: seqs.fna

  3. Metadata file containing the relevant contextual information for each sample: Schisto_microbiome_Metadata.csv

  4. OTU table created using Qiime including all of the taxonomic information: otu_table_mc2_w_tax_no_pynast_failures.biom

Experiment data files

  • Joined and quality filtered (Qiime) sequences
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  • Metadata
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  • OTU table
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  • Qiime 2 analyses
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  • Raw joined sequences
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Latest version

  • Version 2

    2019-10-23

    Published: 2019-10-23

    DOI: 10.17632/y8c7vpc8zp.2

    Cite this dataset

    Jenkins, Timothy; Peachey, Laura; Ajami, Nadim; MacDonald, Andrew; Hsieh, Michael; Brindley, Paul; Cantacessi, Cinzia; Rinaldi, Gabriel (2019), “Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota”, Mendeley Data, v2 http://dx.doi.org/10.17632/y8c7vpc8zp.2

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Institutions

Wellcome Sanger Institute, Manchester University, George Washington University School of Medicine and Health Sciences, University of Cambridge, Baylor College of Medicine

Categories

Microbiome, Mouse, Schistosoma, Schistosomiasis

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

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