RRM1 and PAB domains of translation initiation factor gamma, Tif4631p play crucial role in the nuclear degradation of export-defective mRNAs in Saccharomyces cerevisiae

Description

The dataset is associated with the experiments addressing the role of functional domains of major translation initiation factor gamma, Tif4631p in the nuclear mRNA surveillance in baker's yeast Saccharomyces cerevisiae. Tif4631p is part of a system called CTEXT, which is known to function as a second co-factor of the nuclear RNA exosome. Together with the nuclear exosome, CTEXT targets and degrades two distinct sets of aberrant nuclear messages and a group of normal messages called special messages. CTEXT thereby controls several known fundamental cellular processes such as Unfolded protein Response (UPR) , cell's ability to respond to glucose/nitrogen stress and telomere maintenance. Furthermore CTEXT is expected to control numerous other cellular processes. notably, CTEXT consists of Cbc1, Tif4631, Upf3. In this study we addressed which functional domains of Tif4631p in the nuclear mRNA surveillance processes to gain an insight into the mechanism of CTEXT function. Our Data shows that the N-terminal RRM1 and PAB domains of Tif4631p plays a crucial role in the nuclear mRNA surveillance by making contact with Rrp6p subunit of the nuclear exosome and using this contact recruit target RNAs to the nuclear exosome for their decay. Furthermore, our analyses uncovered Dbp2p as a novel component of CTEXT.

Steps to reproduce

Construction of yeast strains with different mutants, construction of domain deletion mutants in the TIF4631 gene, RT-qPCR analysis from the RNA samples from this strains.

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