Data of Cytotoxicity of Extracts from Curcuma longa and Zingiber officinale
Description
Uterine cervix carcinoma (HeLa) and human hepatocarcinoma (HuH-7) cell lines were obtained for cytotoxicity testing at different doses with secondary metabolites (curcumin and gingerol) extracted from Curcuma longa and Zingiber officinale, respectively. Cell lines of human hepatocarcinoma and carcinoma uterine cervix were grown with Dulbeco’s Modified Eagle’s Medium-High Glucose and it was enriched with 2 % of fetal bovine serum and added with 1.0 % of penicillin/streptomycin. The cell lines were preserved in liquid nitrogen. Freezing of the cell lines was done by a slow protocol with 90 % of fetal bovine serum and 10 % of dimethylsulphoxide. The freezing process was performed through slow changes of temperature in an ultrafreezer: -20 °C for one hour, -80 °C for 24 hours and the last step was performed into the nitrogen liquid. For the assays we used wells cultures of 96 microplates, each assay consisted of 15,000 HuH-7 and HeLa cells, respectively. On the well suspensions, were added the extract doses of C. longa and Z. officinale and blue trypan in order to assess the data of cell viability with methanethiosulfonate (MTS).