Data for a research article "Algal laboratory evolution at a cold temperature leads to overproduction of lipid under both cold and warm conditions"
Description
We working with Chlorella sorokiniana isolates from an earlier laboratory evolution experiment, in which algal populations have evolved under either constantly benign (25°C) or constantly cold conditions (15°C). The growth performance and lipid content per cell of each algal isolate were measured at both temperatures. We measured the growth rate, cell density, chlorophyll-a content, lipid yield, and lipid content per cell of each isolate and ancestral strain at 15 ℃ and 25 ℃ in the laboratory. The selection response values for each trait were calculated. Selection response values (log response ratio) were calculated as standardized measures of evolutionary changes relative to the ancestral strain. We identified that the specific combination of "cold-evolved" algae cultivated under "benign" conditions yields extremely high population-level lipid productivity. Besides, adaptation to cold did not incur a cost in benign environments, and increased lipid content was not associated with a reduced growth rate.
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Institutions
- Beijing Normal University