circRNA array profiling in mouse E18 whole brain following prenatal alcohol exposure.
WT C57BL/6J mice derived from Jackson Laboratory were maintained on a reverse 12 h dark/light schedule (lights on at 8:00 P.M.) in single-housed cages. We utilized a well-validated mPAE paradigm (Brady et al., 2012) that involves giving adult female mice access to either to a solution of either 10% (w/v) ethanol and 0.066% (w/v) saccharin or 0.066% (w/v) saccharin (control) for four hours per day. After validating consistent drinking, mice were given access to these solutions during mating and for the whole duration of their pregnancy. They were euthanized at the end of their pregnancy and whole brain samples were extracted from embryonic day 18 (E18) male and female pups and used for total RNA extraction. Mouse arraystar circRNA profiling service was then used to measure circRNA expression in 12 such samples. 6 samples were from mice that were exposed to Prenatal Alcohol exposure (PAE) and 6 from control mice whose mothers consumed saccharin (SAC).