Dataset on human NORMAL DIAMETER abdominal aorta biomechanics (uniaxial) and histology (specimens harvested during autopsy)

Published: 3 March 2021| Version 4 | DOI: 10.17632/yfj4wfszbw.4
Contributors:
Vivian Carla Gomes,
Erasmo Simão da Silva,
Luiz Fernando da Silva,
Jorge Gomes,
Madhavan Lakshmi Raghavan ,
Michele Alberto Marques,
Alexandre Queiroz,
Gina Silvestre,
Selene Zyngier,
Timothy Kwang-Joon Chung

Description

The present dataset is a collection about the biomechanical behavior and histological characterization of normal diameter abdominal aortas harvested during the autopsy procedure. The primary hypothesis of the present research is: Do cadaveric abdominal aortic walls, when previously stressed by inflation, conserve significant resistance against tearing comparable to no previously stressed aortas described in the literature? Thirty normal diameter abdominal aortas were carefully dissected and had their branches ligated with cotton or prolene sutures. Each specimen has been submitted to intraluminal pressurization, up to the rupture of their wall. This pressurization was made through the inflation of an air balloon inside the specimens up to their rupture. From each border of the rupture site, and from the proximal (control sample 1) and distal (control sample 2) portions of each vessel, samples were harvested for uniaxial tensile tests and histological analysis whenever possible. The uniaxial tensile test utilized the INSTRON SPEC 2200 device and was coordinated by INSPEC software and SERIES IX software. The essential variables collected through this test are failure stress, failure tension, and failure strain. Each test generated a graph representing the relationship between stress and strain. The histological analysis included Picrosirius and Voerhoeff stains1. Unfortunately, some samples were lost, especially during histological processing. A quantitative analysis (collagen and elastic fibers percentage of coverage) was made using the software Pannoramic Viewer. Notable findings: Even after being stretched up to their rupture, the specimens conserved some uniaxial biomechanical properties comparable to aorta samples described in the literature2 (which were not submitted to stress before the tensile test). DATA DESCRIPTION: a) Biomechanical Data: For each valid sample test, three documents were generated: 1. Stress X strain graph (all graphs contain a notification in their left upper corner about the failure stress, strain and tension of each sample). 2. Table (excel file containing all the values related to the stress X strain graph 3. A report from the biomechanical test software containing details of the test It is important to highlight that some samples did not produce valid biomechanical tests, so they do not have their results included here. All available files related to valid biomechanical tests of these 30 normal aortas were included in this dataset. b) Histological Data: Four samples were harvested from each aorta, whenever it was feasible.The percentage of coverage of collagen fibers and elastic fibers is expressed in the table "HISTOLOGY - NORMAL AORTAS.xls" in decimal numbers (for example, 0.36 = 36%). Each case has a "histology files" folder containing the images used in the present analysis. Case 27 histology samples were damaged during processing, therefore were not included here.

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