Dataset on human NORMAL DIAMETER abdominal aorta biomechanics (uniaxial) and histology (specimens harvested during autopsy)
Description
The present dataset is a collection of information about the biomechanical behavior and histological characterization of normal diameter abdominal aortas harvested during the autopsy procedure. The primary hypothesis of the present research is: Do cadaveric abdominal aortic walls, when previously stressed by inflation, conserve significant resistance against tearing comparable to no previously stressed aortas described in the literature? Thirty normal diameter abdominal aortas were carefully dissected and had their branches ligated with cotton or prolene sutures. Each specimen was submitted to intraluminal pressurization, up to the rupture of their wall. This pressurization was made through the inflation of an air balloon inside the specimens up to their rupture. From the border of the rupture sites, and from the proximal (control sample 1) and distal (control sample 2) portions of each vessel, samples were harvested for uniaxial tensile tests, and histological analysis. The uniaxial tensile test utilized the INSTRON SPEC 2200 device and was coordinated by INSPEC software and SERIES IX software. The essential variables collected through this test are failure stress, failure tension, and failure strain. Each sample test generated a graph representing the relationship between stress and strain. The histological analysis included hematoxylin-eosin, Picrosirius, and Voerhoeff stains1. Unfortunately, some samples were lost, especially during histological processing. A quantitative analysis (collagen fibers and elastic fibers percentage of coverage) was made using the software Pannoramic Viewer and Case Viewer. Notable findings: Even after being stretched/stressed up to their rupture, the specimens conserved some uniaxial biomechanical properties comparable to normal diameter aorta samples previously described in the literature by Monteiro e Nynomiya respectively.2,3 DATA DESCRIPTION: a) Biomechanical Data: As explained above, whenever possible, four samples were collected from each specimen destinated to the biomechanical test. It is important to highlight that some samples did not produce valid biomechanical tests, so they do not have their results included here. For each valid sample test, three documents were generated: 1. Stress X strain graph (all graphs contain a notification in their left upper corner about the failure stress, strain and tension of each sample). 2. Table (excel file containing all the values related to the stress X strain graph 3. A report from the Biomechanical test software containing details of the test All available files related to the biomechanical tests of these 30 normal aortas were included in the present dataset. b) Histological Data: The percentage of coverage of collagen fibers and elastin fibers is expressed in the table "HISTOLOGY - NORMAL AORTAS.xls in decimal numbers (for example, 0.36 = 36%). Four samples were harvested from each aorta, when it was feasible.