Stepwise Requirements for Polymerases Theta and Delta in TMEJ (Untreated samples)

Published: 7 August 2023| Version 1 | DOI: 10.17632/ymrs93b9c9.1
Contributor:
Susanna Stroik

Description

Coordinate data for STORM performed in association with DOI: https://doi.org/10.21203/rs.3.rs-2057977. Untreated RPE1 cells expressing a HALO-tagged POLQ. 53BP1 was imaged in green, HALO-POLQ in red, EdU in dark red, and POLD1 in blue.

Files

Steps to reproduce

Description of files: Results file - coordinate maps for each protein Frm1 file - image file ROI - Region of interest for analysis Color assignment: Blue = POLD1 antibody staining Green = 53BP1 antibody staining Red = POLQ-HALO ligand staining Dark red = EdU to identify S phase cells (not included in analysis)

Institutions

New York University, University of North Carolina at Chapel Hill

Categories

Microscopy, Storm

Licence