Mapping data for HTS assembled genomes
Description
There was no significant difference in the diversity of viruses infecting pumpkins in the different sub-regions in Uganda. To undertake this study, High Throughput sequencing was used to determine the diversity of viruses infecting pumpkin. To carry out this study, sixty-five symptomatic and 10 asymptomatic samples were collected and organized into five groups of 15 leaf samples were preserved in RNAlater® solution at -20°C while in transit to the Agricultural Research Council (ARC) in the Republic of South Africa for HTS. Raw reads from the Illumina HiSeq sequencing platform were assembled using the Genome Detective 1.132 bioinformatics pipeline (https://www.genomedetective.com/app/typingtool/virus/; May 20, 2024). Furthermore, the genome isolates were mapped on existing genomes in the GenBank to determine the identity levels.
Files
Steps to reproduce
The HTS assembled Moroccan watermelon mosaic virus genome isolates assembled from the pooled pumpkin leaf samples were mapped on some genome isolates in GenBenk and the resultant mapping information is saved here.