Nuclear pore complex proteins are involved in centromere distribution Ito et al.

Published: 2 April 2024| Version 1 | DOI: 10.17632/yp9sxcn4b4.1
Nanami Ito, Takuya Sakamoto, Hikaru Sato, Yuki Sakamoto, Sachihiro Matsunaga


The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker of the nucleoskeleton and cytoskeleton (LINC) complex. However, it is likely that other nuclear membrane structures and nuclear proteins are involved in centromere dynamics and distribution. Here, we focused on the nuclear pore complex (NPC), which is known to regulate gene expression, transcription memory, and chromatin structure in addition to transport between the cytoplasm and nucleoplasm. We report here that some nucleoporins (Nups) including Nup85, Nup133, Nup43, CG1, Nup93b, and NUCLEAR PORE ANCHOR (NUA) are involved in centromere scattering in Arabidopsis thaliana. In addition, the centromere dynamics after metaphase in the nup85 mutant was found to be similar to that of the condensin II mutant. Furthermore, both biochemical and genetic approaches showed that Nup85 interacts with the LINC complex. These results suggest that Nups regulate centromere scattering cooperatively with condensin II and the LINC complex.



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