Identification and diagnosis of possible mutations in human P-53 lymphocytes

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Data description It was discovered in the last few years that the production of some percentage of mutant p-53 proteins, with the increased stability in type B lymphocytes, leads to the carcinogenesis process. In the full physical examination of patients diagnosed with Chronic Lymphocytic Leukemia were encompassed all patients which have been admitted to hospital with symptoms and clinical features. Male results: Protein concentration in p-53 / µg / dL: 20, 15, 18, 40, 10, 12, 14, 60, 30, 10, 13, 15, 5, 10, 15, 12. Women's results; Protein concentration p-53 / µg / dL: 140, 30, 13, 10. Normal values of normal cell lines on equipment: ELISA = 0.25-0.5 µg / dL, or 2.5- 5ng/mL, [Figure 5]. Very high pathological values in the 3 cases of p-53 were calculated in 2 men in the value of 60 µg / dl, respectively at 40 µg / dl, and in the case of females it was calculated in the amount of 140 µg / dl, the frequency chronic lymphocytic leukemia with transformation into Diffuse Large Lymphoma, (DLL). Statistical interpretations: the concentration of p-53 protein, in the 17 cases with p-53 protein expression, after excluding the 3 out-line cases present in the study, was calculated at the average value of 16.76µg / dl, with STDEV = 8.35, CV = 0.5% and the probability index (NORMDIST) p, was calculated in the value of p = 0.034. The concentration of p-53 protein, in the 17 cases with p-53 protein expression, after excluding the 3 out-line cases present in the study, was calculated at the average value of 16.76µg / dL. In the CLL stage III/IV (n = 3 patients (Table 1), the percentage of p53 isoform protein with elevated values was present in 15% (3 of 20 cases), such as 2 Men = 50μg / dL and 60μg / dl, respectively and 1 Female = 140μg /dL, p value 0.034; Hematological parameters in peripheral blood were changed with the Leucocytes leukocyte number = 250-500 x 10 3 /dL, Hb = 8.6g / dl, Thrombocytosis = 45x10 3 / dL, Lymphocytes in peripheral blood= 85-90%, ( p value = 0.05). In some international studies, the immunological characteristics of patients with CLL having p-53 protein positive, were measured by immunohistochemistry, (IHC). In the second part of the study CLL on the stage I/II, (n=47 patients), the P-53 protein isoform concentration in reactive Lymphocyte B, the average p-53 protein concentration in CLL was on average 47 U/m with 16.7% samples studied, (7 out of 42 cases), Hematological parameters in peripheral blood, Leucocyte number = 35x10³ / dl, Hb = 12,2g / d, Thrombocyte = 140 x x10³ / dL, Lymphocytes in peripheral blood = 75-80%. The average p-53 protein concentration in CLL was on average 47 U/m, in CLL on the stage III/IV (n = 140 patients] and percentage of p53 isoform varied from 7-32%.; Hematological parameters in peripheral blood where changed in Leukocytes number = 350 x10³ / dL, Hb = 10,8g / dL, Thrombocytes = 80 x 10³ / dL, Lymphocytes in peripheral blood = 80-90%; Percent p-53 positive was in percent of 15% 10.

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