Raw high-throughput sequencing data for determining transcription fidelity by E. coli RNAP

Published: 14 February 2023| Version 2 | DOI: 10.17632/ysc6r3dz2m.2
Liang Meng Wee,


We performed in vitro transcription with E. coli RNAP in the absence (−coupling) or in the presence (+coupling) of the ribosome in 2 independent reaction conditions (fidelity and pyrL) and extracted the transcripts for MiSeq. In all, there are 4 datasets, which are single-end reads and each library is equipped with its unique pair of i5 and i7 indexes (See Table S5). The final tabulation (excel files) compares and tabulates errors committed by RNAP in −coupling and in +coupling conditions for fidelity and pyrL transcription assays. Codes for analysis are available at https://zenodo.org/record/6534021#.YntNty8Rrxg (DOI:10.5281/zenodo.6534021).



University of California Berkeley


Biochemistry, Biophysics, Transcription, RNA Polymerase, Coupling Reaction, Cryo-Electron Microscopy, Ribosome, Translation (Protein Synthesis), Single Molecule Method, Force Measurement, Biomolecular Structure Determination