Attachment of Osteoblastic MC3T3-E1 Cells on Diamond-Like Carbon-Coated Silicon Plates

Published: 25-05-2021| Version 1 | DOI: 10.17632/yt52fw55wx.1
Contributors:
Yoshinobu Manome,
Toshiaki Tachibana,
Masanori Hiratsuka,
Keisuke Sato,
Yasuharu Ohgoe,
Kenji Hirakuri

Description

Data were collected by experiments in which DLC was coated on the silicon plate surface, then mouse osteoblastic MC3T3-E1 or fibroblastic NIH3T3 cells were cultured with the coated plates. Cell proliferation by a colorimetric assay and morphological observations using a field emission scanning electron microscope (FE-SEM) were examined. Data include an advantage of the proliferation of osteoblastic cells on DLC-coated plate and visualized images of osteoblastic cell on noncoated or DLC-coated silicon plate. Figure 1. Proliferation of NIH3T3 cells (a) or MC3T3-E1 cells (b) on noncoated or DLC-coated silicon plate. After attachment of the cells on each plate, the proliferation of the cells was assessed on days 3 and 7 post-inoculation. Figure 2. Raw Data Set of FE-SEM pictures of MC3T3-E1 cell. Attached cell on noncoated (a) or DLC-coated (b) silicon plates were observed by FE-SEM. Figure 3. Data Set of flat cross section of MC3T3-E1 on noncoated silicon plate. Figure 4. Data Set of flat cross section of MC3T3-E1 on DLC-coated silicon plate. All the separated pictures and condition of each picture taken are included in the data set. A detailed description of data could be found in the “Data in Brief” article “Manome Y., Tachibana T., Hiratsuka M., Sato K., Ohgoe Y., Hirakuri K. Osteoblastic MC3T3-E1 Cells on the Surface of Diamond-Like Carbon-Coated Silicon Plates: Field Emission Scanning Electron Microscopy Data", in 2021, submitted.

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