Data for: DNA damage in liver cells of the tilapia fish Oreochromis mossambicus larva induced by the insecticide cyantraniliprole at sublethal doses during chronic exposure
This paper described the acute and chronic toxicity of cyantraniliprole to juvenile tilapia was evaluated in the present study. The results showed that 96 h LC50 of cyantraniliprole to tilapia was 38 mg/L. After exposure for 28 days, specific growth rates of the blank control, solution control, and the treatments of 0.037, 0.37 and 3.7 mg/L of cyantraniliprole were 1.14, 0.95, 0.93, 0.82 and 0.70% per day, respectively. The results of micronucleus experiment and single cell gel electrophoresis showed that cyantraniliprole damaged DNA in liver cells of tilapia larvae. Quantitative PCR results showed that cyantraniliprole could induce the up-regulation of the genes Ccne 1, Cdk2, Orc1, Ccna 1, Ccnb, Rpa 3 and Fen1 that are responsible for the DNA damage and repair. Among those genes, Ccna1 and Rpa3 were most notably up-regulated. It is therefore proposed that cyantraniliprole causes DNA damage in liver cells of tilapia and activates DNA damage and repair pathways.