Sugarcane data related to nitrogen remobilisation
Description
Research Hypothesis Water deficit affects nitrogen remobilisation in sugarcane (Saccharum spp. cv. CTC9001bt), leading to reduced chlorophyll content and biomass allocation. It is further hypothesized that rehydration following water deficit conditions will enhance recovery. Data Overview The data collected in this study focuses on the effects of varying water availability conditions on nitrogen dynamics, chlorophyll content, and biomass in sugarcane plants. The experiment was conducted in a glasshouse environment, utilising three-month-old sugarcane plants grown in 10L pots filled with a 2:1 mixture of sand and soil. Experimental Design The experimental design was completely randomized, consisting of two water availability treatments: Hydrated Condition (100% field capacity) - plants maintained at optimal moisture levels. Water Stressed Condition (20% field capacity) - plants subjected water deficit, followed by rehydration to 100% field capacity. Data Collection: Data were collected at two key points: Maximum drought stress After rehydration (following a return to 100% field capacity) Parameters measured included: Nitrogen isotope labeling: Using ¹⁵N-enriched ammonium sulfate, nitrogen distribution within the plant was analysed. Chlorophyll content: Measured using a portable chlorophyll meter (ClorofiLog®). Dry mass: Determined by oven-drying plant parts and weighing them. Notable Findings Nitrogen Distribution: Isotope analysis revealed distinct patterns of nitrogen distribution in plant organs. Under drought stress, there was a notable decrease in nitrogen allocation to leaf structures, which is critical for photosynthesis and overall plant vitality. Data Interpretation The data collected can be interpreted to provide insights into the physiological responses of sugarcane to drought stress and subsequent recovery. Water deficit: The significant reduction in nitrogen remobilisation during drought suggests that sugarcane may face challenges in nutrient availability under water-limited conditions. This can affect growth and yield, particularly in regions prone to drought. Recovery Strategies: The findings emphasize the importance of timely rehydration in mitigating some adverse effects of drought. Understanding the timing and extent of water availability can guide agricultural practices to enhance sugarcane resilience. Research Applications: The data can be utilized to inform breeding programs focused on developing drought-resistant sugarcane varieties. Additionally, the methodologies used for nitrogen isotope labeling can be applied in other studies to investigate nutrient dynamics in different crops.