Cenobamate exhibit prophylactic and therapeutic efficacy against cisplatin-induced peripheral neuropathy in rats: Behavioral and mechanistic insights
Description
This dataset contains behavioral and immunohistochemical data from the study "Cenobamate exhibits prophylactic and therapeutic efficacy against cisplatin-induced peripheral neuropathy in rats: Behavioral and mechanistic insights." Behavioral assessmentS in cisplatin-treated rats include: - *Sensory*: mechanical allodynia (dynamic plantar aesthesiometer), cold allodynia (acetone drop test), thermal hyperalgesia (Hargreaves test) - Motor: rotarod performance and footprint gait analysis -Immunohistochemical analyses report H-score quantification of BDNF, cleaved caspase-3, and IL-1β in dorsal root ganglia and sciatic nerve tissues. Excel files contain raw and processed values for statistical analysis across prophylactic and therapeutic protocols, comparing cenobamate with cisplatin-only and gabapentin-treated control groups.
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Data were generated using a preclinical rat model of cisplatin-induced peripheral neuropathy (CIPN) to evaluate the prophylactic and therapeutic effects of cenobamate. Peripheral neuropathy was induced in adult male Sprague–Dawley rats by intraperitoneal administration of cisplatin (3 mg/kg/week) for five consecutive weeks. Two experimental protocols were implemented: a prophylactic protocol, assessing preventive effects during cisplatin exposure, and a therapeutic protocol, assessing treatment effects after neuropathy establishment. Behavioral assessments included standardized sensory and motor function tests. Mechanical allodynia was measured using a dynamic plantar aesthesiometer, recording paw withdrawal latency (PWL) and paw withdrawal threshold (PWT). Cold allodynia was evaluated using the acetone drop test, and thermal hyperalgesia was assessed using the Hargreaves plantar test. Motor coordination and balance were evaluated using the rotarod test, while gait analysis was conducted using the footprint test to measure stride length. After behavioral testing, animals were euthanized, and dorsal root ganglia (DRG) and sciatic nerve (SN) tissues were collected for histological and immunohistochemical analysis. Immunohistochemistry was performed to assess expression of brain-derived neurotrophic factor (BDNF), cleaved caspase-3, and interleukin-1β (IL-1β). Staining intensity and the percentage of positive cells were quantified using the H-score method. Raw measurements were recorded in Microsoft Excel spreadsheets and analyzed using SPSS software. All procedures and instruments followed established experimental protocols widely used in neuropathy research.