The history of genetic diversity and effective population size of isolated Microtus oeconomus population on Kis Balaton

Published: 3 March 2021| Version 1 | DOI: 10.17632/yx2zmhmxkc.1
Contributor:
Veronika Sládkovičová

Description

The distribution of many species is fragmented due to human activities and isolated populations are often affected by genetic changes. Small populations are endangered by inbreeding depression, often leading to a low mean fitness of small populations and to an increased extinction risk. Using museum samples of protected Microtus oeconomus mehelyi from the years 1950, 1964 and 2000, we studied the population which inhabits the Kis Balaton area that has considerably changed by human activity in the last centuries. - sampled a total of 56 skin specimens which were collected in 1950 (32 specimens) and 1964 (24 specimens) -Samples washed in PBS overnight, put in sterile water for one hour and then cut into small pieces. - the QiaAmp DNA Mini Kit and DNeasy Blood & Tissue Kit (Qiagen) - In the Hungarian Natural History Museum - isolated DNA samples of M. oeconomus from Kis Balaton (collected in the years 1999 and 2000)- we used 26 of these samples. - Imicrosatellite genotyping using 14 microsatellite loci in 3 multiplex sets (published in Sládkovičová et al., 2018) using the touch-down PCR protocol - the multiple tubes approach - marker Ma54 rejected See also file "Read me"

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Institutions

Hungarian Natural History Museum, University of Pecs Biologia Intezet

Categories

Microsatellite DNA

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