Gill, hepatopancreas, nervous gland, muscle and gonad lC-HRMS-based metabolomics of mature male and female Palaemon serratus
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We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. The data were dereplicated using specific metabolomic software (MetaboScape 4) and 2,782 features were extracted, 1,720 of them being also analyzed on MS/MS mode, supporting molecular networking investigations with Metgem 1.3.6. These metabolites were thus attemptedly identified using GNPS databases for de-novo annotation followed by manual curation.