CL316,243 induced thermogenesis in adipose tissue of C57Bl/6J mice
Brown adipose tissue (BAT) thermogenesis confers beneficial effects on metabolic diseases such as obesity and type 2 diabetes. Nevertheless, the mechanism and lipid driving the process that evokes this response have not been investigated yet. Here, utilizing a multi-omics approach of integrative transcriptomic and lipidomic to explore the mechanism of regulating thermogenesis in BAT and providing promising lipid biomarkers and biomarker genes for thermogenic activators as anti-obesity drugs. Lipidomics analysis demonstrated that a high abundance of glycerophospholipids and sphingolipids was more significant in BAT than WAT. Enrichment analysis of upregulated DEGs between WAT and BAT screened suggested the differences were mainly involved in lipid metabolism. Besides, β3-adrenergic agonist stimulation reduced the levels of TAG and DAG, increased the content of PC, PE, CL, and LPC, and expression of genes involved in thermogenesis, fatty acid elongation, and glycerophospholipids metabolism in BAT. In this study, based on interpretating the inherent characterization of BAT as thermogenic tissue through comparison with WAT as fat storage tissue, adrenergic stimulation-induced BAT thermogenesis further identified specific lipid biomarkers (7 TAG species, 10 PC species, 1 LPC species, and 1 CL species), and Elovl3 and Crat gene biomarkers which may provide targets for combating obesity by boosting BAT thermogenesis.