Data for: Evaluation of decalcification for recovery of DNA from bone

Published: 4 Oct 2017 | Version 1 | DOI: 10.17632/z5vgzjc2gm.1
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Description of this data

The effect of decalcification on pulverised bone samples was studied to evaluate its value when processing reasonably good quality samples. The decalcified and non-decalcified bone samples were extracted using phenol-chloroform extraction method, quantitated with GoTaq® qPCR Master Mix (Promega®, UK) using Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific®, UK) and amplified using an in-house amplification multiplex. The results showed that the decalcification of bone samples is not a necessary when processing good quality samples and higher DNA yields were obtained without the decalcification process. The electropherograms produced from the extracted DNA samples without decalcification were good quality and displayed no PCR inhibition.

Experiment data files

This data is associated with the following publication:

Evaluation of decalcification for recovery of DNA from bone

Published in: Forensic Science International: Genetics Supplement Series

Latest version

  • Version 1

    2017-10-04

    Published: 2017-10-04

    DOI: 10.17632/z5vgzjc2gm.1

    Cite this dataset

    Iyavoo, Sasitaran; Goodwin, William; Hadi, Sibte (2017), “Data for: Evaluation of decalcification for recovery of DNA from bone”, Mendeley Data, v1 http://dx.doi.org/10.17632/z5vgzjc2gm.1

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Categories

DNA, Bone, Decalcification

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The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

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This dataset is licensed under a Creative Commons Attribution 4.0 International licence. What does this mean? You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

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