Lacticaseibacillus rhamnosus MDC 2012 whole-genome

Published: 22 August 2025| Version 2 | DOI: 10.17632/z6g7hw2sfh.2
Contributor:
Artur Hambardzumyan

Description

Genetic and Biochemical Characterization of Lacticaseibacillus rhamnosus MDC 2012 and Its Potential in Processing of Raw Pork Meat

Files

Steps to reproduce

Whole-genome sequencing was performed following the recommendations of Oxford Nanopore Technologies (UK), using a Flongle R10.4.1 flow cell and the ligation sequencing kit SQK-LSK114, according to the protocol specified in the g-dna-by-ligation-sqk-lsk114-document (document: Flongle-en-GDE_9161_v114_revY_30Jan2025.pdf). High-molecular-weight, high-purity DNA was isolated using a classic phenol-chloroform extraction method. The raw sequencing data were processed using the Oxford Nanopore Technologies EPI2ME (EPI2ME Desktop V5.2.3) Bacterial Genomes workflow.

Institutions

Armbiotechnology Scientific and Production Center

Categories

Microbial Ecology of Fermented Meat Products

Funding

Ministry of Higher Education Science, and Technology

Research Project № 21AG-2I090

Licence