Topology recapitulates ontogeny of dendritic arbors

Published: 26 June 2023| Version 1 | DOI: 10.17632/z97bs2c8p7.1
Maijia Liao,


Drosophila melanogaster larvae were raised on glucose fly food at 25℃. 96hr AEL larvae were used for all analyses. For visualization of dendritic morphologies of class III neurons (ddaF), nompC-Gal4 (Stock 36361 from Bloomington Drosophila Stock Center) was crossed with 10XUAS-mCD8::GFP (32187). The fly strain ppk-cd4-tdGFP (a gift from Han Chun (Cornell University)) was used for imaging class IV neurons. The imaging was done using a spinning disk microscope: the Yokogawa CSU-W1 disk (pinhole size 50 μm) built on a fully automated Nikon TI inverted microscope with perfect focus system, an sCMOS camera (Zyla 4.2 plus sCMOS), and running Nikon Elements software. Individual neuron image stacks were acquired with a 60X 1.2 NA water immersion lens with a z step size 0.16 μm. Raw images for class IV and class III neurons were uploaded. Raw images exist in .nd2 or .tiff forms. Due to the size limit, raw images for class III neurons were processed using maximum intensity z-projection and pairwise stitching plugins of ImageJ software. This manuscript also contains datasets from Drosophila Hemibrain, mouse visual cortex and Neuromorpho. The link to hemibrain dataset is: The link to electron microscopy dataset on layer 2/3 of the mouse visual cortex is: 301 neuron reconstructions with spine information were stored in microns_example_all.mat. The link to neuromorpho database is: Here we used the neuromopho dataset from the paper (Cuntz, H., Bird, A. D., Mittag, M., Beining, M., Schneider, M., Mediavilla, L., ... & Jedlicka, P. (2021). A general principle of dendritic constancy: A neuron’s size-and shape-invariant excitability. Neuron, 109(22), 3647-3662.).



Yale University


Neuroscience, Biophysics