Dr. MOHAMMAD HAZAIMEH
Description
The phytochemical screening of both products was run following the standard methods. Polar extracts of both B. aegyptiaca fruits and A. sativum bulbs were prepared to test their inhibition activities on E. coli stain through agar well diffusion method for 72 hours.
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: Samples were (1) Balanites aegyptiaca fruits, (2) Alium sativum bulbs, the collected samples were cleaned, let to dry under shade, then crushed and extracted with acetone which later evaporated under room temperature leaving behind dry extract which were used to prepare the aqueous extracts used for run GC-MS, phytochemical screening and the mocrobial inhibition test (through 5 different concentrations) (3) E. Coli strain Was brought from the Central Laboratory and cultured on selective medium: Eosin Methylene Blue (EMB) Agar at 39:1000 (g:ml water) in sterilized plastic Petri-dishes (each contain about 10-15 ml of EMB medium) Inhibition activities of the prepared plant aqueous extract on E. Coli strain were run through agar well diffusion method. E. coli samples were streaked on the Petri-dishes, and immediately, on the center of each Petri-dish, a small disc paper (4 mm diameter) which impregnated for 5 minutes in one of the prepared concentrations was placed, then incubated for 72 hours at 37oC. 1- The qualitative phytochemical output 2- The qualitative and quantitative GC-MS output 3- The inhibition diameter (mm) for each concentration for each extract compred to negative and positive control the obtained data of the phytochemical and GC-MS analysis were not subjected to any kind of analysis, but the data obtained from the inhibition activity tests were subjected to simple descriptive statistics (mean and SE) and to the least significant differences (LSD) analysis