Metabolic effects of exhaustive single leg exercise in relation to the ACE-I/D gene polymorphism

Published: 25 July 2022| Version 1 | DOI: 10.17632/zb8jptj6tv.1
Martin Flück,


The data reports the physiological characteristics of healthy subjects, aerobic fitness and the insertion/deletion polymorphism of the angiotensin converting enzyme gene, as well as data for blood metabolites before and after exhaustive one-legged exercise.


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Healthy, male white Caucasian subjects were recruited from White British men of the Greater Manchester Area. Exclusion criteria were smoking, long-term ill-health, an age below 18 years or over 40 years, and a relative VO2max below 40 mL O2 min -1 kg-1 or above 60 mL O2 min -1 kg-1 (as determined post-hoc). Subjects reported to the laboratory on two occasions to estimate aerobic capacity during one session of two-legged cycling exercise and one session of exhaustive one-legged cycling exercise in the fasted state in the morning to test the metabolic response. The two visits were separated by at least 2 days. Oxygen uptake, respiration exchange ratio (RER), and the blood concentrations of selected metabolites, i.e. glucose, triacylglycerol (TAG), total cholesterol (T Cholesterol), low- (LDL) and high-density lipoprotein (HDL), total ketone (ketones), were monitored in samples being collected prior to, and ½, 3, and 8 hrs after exercise. Concomitantly, biopsies were collected from m. vastus lateralis of the non-exercised leg immediately before exercise, and ½ ,3, and 8 hrs after exercise from the exercising leg, respectively. Between the first and last biopsy subjects rested under supervision without physical activity reading a book, watching a movie, listening to music, or surfing the web, and consuming a meal based on sandwiches and water, if desired. The biopsy material was used to quantify muscle capillarisation, and the concentration of glycogen. In the two-legged exercise test, the aerobic fitness state was determined post-hoc based on VO2max values below (unfit) or above (fit) 50 mL O2 min -1 kg-1, respectively. The ACE-I/D genotype was determined, in a double-blind manner, with allele specific polymerase chain reactions on genomic DNA being isolated from a buccal swap collected before the one-legged exercise.


Manchester Metropolitan University


Human Physiology