Dataset of biochemical parameters of tear fluid in chemical burns

Published: 5 August 2021| Version 2 | DOI: 10.17632/zhk775h6n4.2
Contributor:
Behruz Oralov

Description

The article presents the data of examinations of patients being treated in the emergency ophthalmomicrosurgical department of the Clinical Hospital of Emergency Medical Care of the city of Tashkent, Uzbekistan. 110 patients (155 eyes) with chemical eye burns were randomly divided into three homogeneous groups. The first of them, the comparison group, received traditional therapy according to ophthalmological standards. I main groups on the basis of conventional therapy received PDT method. II main group therapy, along with the main group I was added to eye drops Methylethylpiridinol hydrochloride. The patients underwent biochemical studies of the antioxidant activity of the lacrimal fluid. All patients underwent a determination of biochemical parameters of tear fluid. These data are necessary for researchers to develop diagnostic criteria and observe the dynamics of the effectiveness of the treatment of chemical burns of various etiologies. The data is also useful for ophthalmologists when determining the severity of a chemical burn and choosing a suitable complex treatment.

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Biochemical studies of tear fluid were performed in 60 patients (60 eyes) of three groups with I-III degree of chemical burn and 10 practically healthy individuals (10 eyes). From the affected eye of patients, tear fluid was taken by cannula from the lacrimal lake, observing the required rules according to the method of Terekhina N. A. before and after treatment. The resulting tear fluid was placed in a centrifuge tube and centrifuged at 1500 rpm for 5 minutes (Mass spectrometry, spectrofotometr SF-46.). The level of malondialdehyde (MDA) and NO system was determined by the sum of the metabolites of nitrates and nitrites ( NO, ONOO -) (Golikov P. P. et al. in the modification of Komarin A. S., Azimov R. K.), as well as the activity of superoxide dismutase (SOD) (Dubinina E. E. et al.) and catalase (CT) (Koralyuk M. A. et al.). Also, the level of acidity (pH) in the lacrimal fluid was determined using a special pH meter.