The data of study about the chitosan-selenium nanoparticles on depression-like behaviour induced by fluoride in mice via JAK2-STAT3 pathway

Published: 6 August 2021| Version 1 | DOI: 10.17632/zj4k8j5gj9.1
Contributor:
Jinming Wang

Description

These data demonstrated that 1 mg/kg·bw CS-SeNPs mitigated fluorine-induced cortical pathological structural (Raw data 25) and functional damage (Raw data 26), abnormal activation of microglia (Raw data 27-32), and enhanced nuclear localization of P-STAT3 (Raw data 33). Raw data 25 and 26 revealed that fluoride induced obviously bigger average area of vacuolation, further the significantly reduced number of cortical surviving neurons. With 1 mg/kg·bw of CS-SeNPs supplementation, the vacuolation damage and the reduced Nissl body in the fluoride group was improved, suggesting that appropriate concentration of CS-SeNPs alleviated the oxidative stress further promoted the survival of neurons through antagonizing the neurotoxicity of fluoride. Raw data 27-29 demonstrated that fluoride could activate microglia in cortex mainly manifested as reduced number of ramified microglia and increased number of amoeboid microglia. Raw data 30-32 showed that F treatment lowered the Ramification Index, the average branch length and raised the Solidity index. After 2 mg/kg·bw CS-SeNPs treatment alone, the Ramification Index was significantly reduced, and the Solidity was significantly increased, indicating that the supplement of 2 mg/kg·bw CS-SeNPs caused the overactivation of microglia to a certain extent in Raw data 30-32. After the fluorosis mice were gavaged with 1 mg/kg·bw of CS-SeNPs, the number of ramified microglia in the cortex increased, amoeboid microglia decreased (Raw data 27-29), and average branch length, Solidity, and Ramification Index parameters recovered to normal levels (Raw data 30-32). Raw data 27-32 showed that F and 2 mg/kg·bw CS-SeNPs could cause the excessive activation of microglia, and intragastric administration of 0.5, 1 mg/kg·bw CS-SeNPs can inhibit the abnormal activation of microglia by restoring the characteristics of Solidity and Ramification Index of microglia. Raw data 33 demonstrated that the p-STAT3 nuclear localization of mice microglia induced by fluoride was increased by 42.54%±0.499. However, the increased p-STAT3 nuclear localization ratio in the F group was blocked by the supplementation of CS-SeNPs at 1 mg/kg·bw. These data provide pathological data to support the neurotoxicity of high doses of nano-selenium (2 mg/kg·bw CS-SeNPs). The lower dose of 0.5 mg/kg·bw CS-SeNPs still provided a safe threshold for long-term consumption of nano-selenium.

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This was how we get the data: Raw data 25:After the perfusion, the whole-brain tissue was placed in 4% paraformaldehyde (Beijing Solarbio Science & Technology Co., Ltd.) for fixation. After fixation for a period of time (33 h), the brain tissue was transferred to 75% alcohol for preservation at 4°C (preservation for no more than 1 month). Under a microscope (Olympus, Tokyo, Japan), observe morphological changes and product the histopathological examination. Under a microscope of 400x, three visual fields with non-overlapping cortical areas were randomly selected, and their vacuolation areas were counted by Figi Image J software, and their average values were taken. Three successive slices were taken from each sample and counted separately. Every group had three samples. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 26: Under 400x microscope (Olympus, Tokyo, Japan), the number of living neurons was counted in three randomly selected fields with non-overlapping cortex, and the average value was taken. Three successive sections were taken from each sample, and the average value was taken as the number of cells in a sample. Each group had three samples. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 27-29: Iba-1 positive cells were counted at multiple of 400x microscope (Olympus, Tokyo, Japan), and the number of microglia was counted in 5 fields with non-overlapping cortex. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 30-32:The staining method is the same as Raw data 27-29. IBA1-immunopositive microglia pictures (400x) converted to the Binary profifiles with Fiji Image J software. Binary profifiles converted to the corresponding 2D trace, and the microglia activation index was calculated as follows: Solidity = microglia cell area/Convex hull area (1) Ramification Index = terminal segments number / primary processes number (2) Image depicting concentric circles imposed upon cells at 2.5 μm intervals to calculated the Length of the microglia branch. The raw data were analysed by the GraphPad Prism 6.01 software. Raw data 33: Under a 200x microscope (Olympus, Tokyo, Japan), and p-STAT3 nuclear staining in microglia (%) was counted in 4 fields with non-overlapping cortex. The count for each field is the average of three samples. The raw data were analysed by the GraphPad Prism 6.01 software.