Implications of Lipoprotein (a) [Lp(a)] gene polymorphic sequence variations in VTE
Description
Venous thromboembolism (VTE) is a common disease that involves key genetic element of risk. Potential relationship of Lp (a) gene 93C>T and 121G>A polymorphic sequence variations and its protein expression was analyzed between VTE cases and healthy controls. The present study enrolled 101 VTE cases and frequency matched age and gender 110 healthy controls. Genotyping was done using PCR-RFLP and the protein expression of lipoprotein (a) was estimated by ELISA. Heterozygous genotype ‘GA’ of Lp (a) +121 G>A was significantly more prevalent in controls than VTE cases (OR= 0.41, p=0.002). Also, a significant protective role against VTE risk was observed for combined variant genotype GA+AA whose frequency in controls was significantly higher 64.5% versus 44.6% in cases (OR= 0.44, p=0.003). LP (a) +121 G>A variant genotypes (GA+AA) was significantly found more in controls for males, smokers and subjects with BMI <25 than VTE cases (p<0.05). Significant difference in mean serum Lp(a) levels between VTE cases estimated as 36.41 mg/dl as compared to 32.00 mg/dl in controls (p<0.05). Also significant difference was found in the mean LP (a) levels between cases and controls for 30-70mg/dl (p<0.05). Our study conclude the inverse role of Lp(a) polymorphic variation +121 G>A in the pathogenesis of VTE. Further results support the relevance of serum lipoprotein (a) levels in the pathogenesis of VTE and its probability to serve as an indicative marker for VTE.