Jais et al., Cell 2016 - FIGURE 7C & D
Description
Raw data corresponding to Figures 7C and 7D from Jais et al., Cell (2016), PMID: 27133169. Figure 7C presents the quantification of blood–brain barrier (BBB) permeability in obese APP.PS1-transgenic mice lacking myeloid-derived VEGF. Permeability was assessed by measuring the extravascular accumulation of endogenous immunoglobulin G (IgG). Four experimental groups were analyzed: control animals, APP.PS1-transgenic mice, VEGFΔmyel mice, and VEGFΔmyel/APP.PS1-transgenic mice, all maintained on a high-fat diet (HFD). Figure 7D provides representative confocal images illustrating these findings.
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Quantification of blood–brain barrier (BBB) permeability was performed using Fiji (ImageJ). Confocal images were acquired using identical settings across all experimental conditions. Image analysis proceeded as follows: the lectin channel (C2) and the IgG channel (C3) were each subjected to background subtraction. Threshold values were set to 14/85 for the lectin channel (C2) and 13/85 for the IgG channel (C3). To isolate extravascular IgG accumulation, the lectin channel (C2) was subtracted from the IgG channel (C3) using the Image Calculator function (“C3 SUBTRACT C2”). The resulting image represented IgG signal outside the vasculature and was quantified by measuring the integrated density.