Periphery signals generated by Piezo-mediated stomach stretch and Neuromedin-mediated glucose load regulate the Drosophila brain nutrient sensor
Description
We uploaded the raw data of the manuscript entitled "Periphery signals generated by Piezo-mediated stomach stretch and neuromedin-mediated glucose loads regulate the Drosophila brain nutrient sensor” as an article authored by Yangkyun Oh, Jason Lai, Soohong Min, Huai-Wei Huang, Stephen Liberles, Hyung Don Ryoo, and Greg S.B. Suh.
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1. All the fly lines were raised on standard cornmeal-molasses medium food, which was made by using 940 ml distilled water, 9 g agar, 15 g yeast, 36 g cornmeal, 36 ml molasses, 1.12 g tegosept, 3.8 ml propionic acid for making 1 liter of normal fly food, at 23 °C under the 12 hours light and 12 hours dark cycles. All the lines used for Expresso feeding behavioral assay and the two-choice behavioral assay were backcrossed into the w1118 (Bloomington stock no.6323 or no.5905) background for at least five generations. Fly lines were obtained as described in the key resources table. 2. In vivo and ex vivo calcium imaging using GCaMP6s or GCaMP6f were performed as previously described with minor modifications (Ai et al., 2010; Dus et al., 2015). 3. Immunohistochemistry of various organs (brain, VNC, Crop, foregut, and midgut) was performed as previously described (Oh et al., 2019). 4. The multicolor flip-out method to trace each DH44 neuronal projection was performed as previously described (Nern et al., 2015). 5. The Expresso hardware device that was designed by Nilay Yapici (Cornell University, NY) with the consultation with William Dickson at IO Rodeo was manufactured by Scitech Korea Inc. The feeding assay using the Expresso system was performed as previously described (Min et al., 2021; Yapici et al., 2016). 6. The two-choice behavioral assay was conducted as previously described (Dus et al., 2011). 7. The ex vivo calcium imaging using GCaMP6f or ex vivo membrane potential measuring using ASAP2f were performed as previously described with minor modifications (Oh et al., 2019).