Characterization of the complete mitogenome data of Ischyja marapok (Lepidoptera: Noctuoidea: Erebidae) from Malaysia
This dataset described the complete mitochondrial genome of Ischyja marapok (Lepidoptera: Noctuoidea: Erebidae) from Malaysia, sequenced using the next-generation sequencing technologies. It has a total length of 15,421bp in length, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal rRNAs and a control region. The genomic DNA was extracted from fresh tissue sample of Ischyja marapok using the Qiagen Blood and Tissue Kit (Qiagen, Valencia, CA) and fragmented using a Bioruptor® system. The library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®. The sample was then sent for sequencing using the Illumina NovaSeq 6000 platform with 150 paired-end mode (PE150). The assembly was done using NOVOPlasty v.4.2 and run through a PALEOMIX BAM pipeline to assess the mitogenome mapping. Annotation was done using the MITOS v2 web server and the predicted protein-coding genes were further verified using the Open Reading Frame (ORF) Finder. The annotated mitogenome sequence file was converted into an accepted Genbank format using the GB2sequin web application. The circular mitogenome map was generated using OGDRAW. The mitogenome is available in Genbank under the accession number ON165249.