Raw "origscale" 24-h metabolomics intensity data from 8 different murine tissues under chow or high fat diet
Description
Raw 24-h metabolomic intensity data from 8 different murine tissues: suprachiasmatic nucleus (SCN), medial prefrontal cortex (mPFC), gastrocnemius skeletal muscle, interscapular brown adipose tissue (BAT), epididymal white adipose tissue (WAT), liver, serum, and cauda epididymal sperm. Six week old male C57BL6/J mice were purchased from JAX / Jackson Labs (Stock Number: 000664). Mice were were randomly assigned to experimental groups, maintained on a 12hr light/12hr dark cycle (ZT0 corresponds to lights on and ZT12 to lights off in the animal facility), and fed ad libitum for 10 weeks with either standard chow diet (Prolab RMH 2500) or high fat diet (HFD) composed of 60% Kcal from fat (Research Diets, D12492). Animals were separated into individual cages 1 week before tissue collection. Five male mice for each time point/diet were used. Tissues were immediately collected after cervical dislocation and stored at -80°C until further processing/analysis. Serum was prepared from an abdominal/thoracic blood sample and stored at -80°C. Sperm was collected after swimming out from the caudal portion of the epididymis in non-capacitating media (MEM without BSA) for 10min at 37°C. Non-targeted metabolite profiling, peak identification, and curation was performed by Metabolon (Durham, NC, USA) and by the Genome Analysis Center (GAC), Helmholtz Zentrum München (Neuherberg, Germany). Liver, serum, and sperm were processed and run by Metabolon on an HD3 system using described methods (Abbondante et al., 2016; Eckel-Mahan et al., 2012). Briefly, this analytical system combines a Linear Ion Trap MS/MS (LTQ XL, Thermo Scientific) coupled with UPLC (Acquity, Waters), and consists of 2 reverse phase (RP)/UPLC-MS/MS methods: 1) with positive ion mode electrospray ionization (ESI) optimized for acidic species, and 2) with negative ion mode ESI optimized for basic species. An additional GC/MS platform for volatile compounds was used in parallel. WAT and BAT samples were processed and run by the GAC on the same analytical system, with the exception of the GC/MS platform, and with curation again performed by Metabolon. Skeletal muscle and brain tissues (SCN & mPFC) were processed and run by Metabolon on their HD4 platform, which runs with High Resolution Accurate Mass (HRAM) MS/MS (QExactive, Thermo Scientific) also coupled with UPLC (Acquity, Waters). Overall, we processed and analyzed a total of 70 tissues each of liver, serum, BAT, and WAT (5 replicates x 2 groups x 7 time points, including additional time point ZT24), and 60 tissues each for SCN, mPFC, gastrocnemius skeletal muscle, and sperm (5 replicates x 2 groups x 6 time points). One biological replicate each from chow-fed SCN at ZT20 and from HFD-fed mPFC at ZT4 were lost during sample processing, leaving 4 remaining replicates each for these particular time points/diets. Two biological replicates from chow-fed mPFC at ZT4 were likewise lost, leaving 3 remaining replicates.