Real-Time Cell Analysis Dataset of L929 Cells Treated with Dental Restorative Materials

Published: 4 May 2020| Version 1 | DOI: 10.17632/zr4jfzy78g.1
Contributors:
Türkay Kölüş,
Hayriye Esra Ulker

Description

Cytotoxicity of seven different restoration materials [Fuji IX GP Capsule (GC), Activa BioActive Restorative (Pulpdent), EQUIA Forte (GC), Biodentine (Septodont), Vitrebond (3M ESPE), Fuji II LC Capsule (GC) and Glass Fill (GCP)] were evaluated in vitro by real-time cell analysis method on L929 Mouse Fibroblasts. RTCA v2.0 (ACEA Biosciences) or newer software is required to read this dataset.

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Steps to reproduce

1) Restorative materials to be tested prepared according to manufacturer's instructions 2) Prepared test materials placed in teflon disks and teflon disks covered with glass slabs to ensure the standardized sample size 3) After the primer setting, 7 samples of each test material placed in each well of a 6-well plate. 4) The samples were stored in incubator at 37°C for 24 hours to ensure complete curing. 5) 3 ml of DMEM was added to the wells to ensure that the material surface area to culture medium volume ratio was 91.6 mm2/ml according to ISO standards. 6) Restorative test samples in 6-well plates with DMEM, stored in incubator at 37°C for 24 hours for release its components into the culture medium 7) In addition to the original extract, dilutions with culture medium at ratio of 1/2, 1/4, 1/8, 1/16, 1/32 were prepared and stored with falcon tubes. 8) Pre-warmed 50 µl of DMEM added to each well of E-plate 16. 9) E-plates were placed in the real-time cell analysis station (xCELLigence RTCA DP, ACEA Biosciences) and background reading was done. 10) 100 µl L929 cell suspension at density of 104 cell/ml seeded into each wells of E-plates, except medium control wells. 11) E-plates were kept in the safety cabinet for 30-60 minutes to allow the cells to adhere to the well base. 12) E-plates placed in the xCELLigence RTCA DP station and an CI measurement was taken every hour. 13) Cells adhered to the plate bases and proliferated inside the real-time cell analysis station with 5% CO2 and 95% humidity at 37°C for approximately 24 hours. 14) E-plates were removed from the real-time cell analysis station 15) The medium in the wells was aspirated before the cells were treated with extracts. 150 µl DMEM was added to medium control and cell control wells. 150 µl volume of restorative material extract was added to the other wells at the determined concentrations (at ratio of 1/2, 1/4, 1/8, 1/16 and 1/32). 16) E-plates were returned to the real-time cell analysis station and the device was programmed to take measurements every 15 minutes for 144 hours. 17) The data obtained with real-time cell analysis station were recorded with RTCA Software (v. 2.0.0.1301, ACEA Biosciences) and RTCA experiment dataset was obtained.