Milk fat globule membrane protects Bifidobacterium longum ssp. infantis ATCC 15697 against bile stress by modifying global transcriptional responses

Published: 28 October 2023| Version 2 | DOI: 10.17632/zrb27bzsmz.2
, Lili Zhang


Milk fat globule membrane (MFGM) can protect probiotic bacteria from bile stress. However, its potential mechanism has not been reported. We studied that the viability, morphology and gene transcriptional response of Bifidobacterium longum subsp. infantis ATCC 15697 (BI_15697) stressed by bile salts with or without MFGM. It was shown that MFGM alleviated the reduction in BI_15697 population induced by 0.2% porcine bile stress and restored the population to the control levels. MFGM ameliorated the shrunken, fragmented appearance and irregular morphology of BI_15697 and maintained cell integrity disrupted by bile stress. RNA-seq results showed that MFGM increased transport of glucose and raffinose and decreased that of branched-chain amino acids (BCAA) in the presence of bile salts. MFGM stimulated the expression of genes involved in the synthesis of raffinose in galactose metabolism and the metabolism of BCAA, suggesting that MFGM stimulated the accumulation of raffinose and BCAA in the presence of bile. In addition, MFGM stimulated the expression of two bile efflux transporters under bile stress. Together, the multifactorial response helps BI_15697 excrete bile salts and maintain cellular integrity in response to bile stress. Seven supplementary tables was presented here for this paper.



University of Illinois at Urbana-Champaign, Heilongjiang University of Chinese Medicine, Northeast Agricultural University


Food Microbiology, Transcriptomics, Milk Composition


Key R&D Plan in Heilongjiang Province


National Natural Science Foundation of China


Characteristic Probiotics and New Fermented Food” Team in Northeast Agricultural University