"Cu(II)-based DNA Labeling Identifies the Structural Link Between Activation and Termination in a Metalloregulator."

Published: 18 January 2022| Version 1 | DOI: 10.17632/zrxjrc7fgn.1
Contributors:
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, Sunil Saxena

Description

EPR DEER, CW-EPR, MD simulation, and UV/Vis raw and processed data pertaining to the article "Cu(II)-based DNA Labeling Identifies the Structural Link Between Activation and Termination in a Metalloregulator" in press by ChemSci. DOI: 10.1039/D1SC06563G.

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Methods and experimental details regarding instrumental parameters and sample preparation are available in the Supplementary Information document hosted by Chemical Scientific. (https://www.rsc.org/suppdata/d1/sc/d1sc06563g/d1sc06563g1.pdf). In order to analyze the EPR DEER and CW-EPR data MATLab2020b or newer with DEERAnalysis2021 (https://epr.ethz.ch/software.html) and EasySpin (https://www.easyspin.org/) installed will be needed respectively. Explicit details of parameters used in each software package are outlined in the Supplementary Information.

Institutions

University of Pittsburgh

Categories

Structural Biology, Continuous-Wave Electron Paramagnetic Resonance, Time-Domain EPR

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