Liposome formulation for drug delivery
Given the hydrophobicity of coumarins (OT-55), we used another route of administration by encapsulating our molecule of interest. First generation liposomes are effective because they are close to the composition of biological membranes. A thoughtful choice that made it possible to avoid the phenomena of rejection or hypersensitivity. The proportions for the formulation were studied and showed a saturation phenomenon. To be sure that we had trapped our coumarin synthesis, a panoply of tests were carried out (well detailed in the manuscript), additional data were added in order to characterize our entity. We therefore use ATR-FTIR to demonstrate an interrelation between coumarin and membrane phospholipids.
Steps to reproduce
Attenuated total reflection-Fourier transforms infrared spectroscopy (ATR-FTIR): The IR spectra of phospholipids, cholesterol, trehalose and liposomes loaded with 4-hydroxycoumarin were recorded using an ATR-FTIR at room temperature by Alpha’s Platinum Diamond Brüker. This accessory employed a silicon internal reflection element (IRE) and the standard deuterium triglycine sulfate (DTGS) as detector. Spectra collected using this device represent the average of 32 individual scans possessing a spectral resolution of 2 cm−1. Spectra were analyzed in transmittance mode in a range analysis from 4000 to 400 cm-1.