Untargeted LC-MS/MS-based metabolomics of Ctrl and VIM-overexpressing THP1-derived macrophages

Description

Untargeted LC-MS/MS-based metabolomics was applied to Ctrl and VIM-overexpressing THP1-derived macrophages (M0 status), which was done in Novogene company (Bejing, China). Raw_files-X101SC23122028-Z01-J001-B1-42.zip was the raw results. Result-X101SC23122028-Z01-J001-B1-42.zip was the routine analyzing results reported by the company.

Steps to reproduce

THP1 are cell lines of monocytes from American Type Culture Collection (ATCC), which were grown in RPMI-1640 (Gibco, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, CA, USA) and 1% Penicillin-Streptomycin at 37℃ with 5% CO2. All the cell lines are routinely tested for Mycoplasma contamination every 3 months. For macrophage differentiation, THP1 were firstly treated with 20 nM Phorbol 12-myristate 13-acetate (PMA, S7791, Selleck, China) for 24 h, then washed by RPMI-1640 for one time. After recovering in culture medium for 48 h, the cells were washed with PBS for two times and then scraped from the culture plate. After centrifuging 1000g for 1 min, the supernatant was removed . The pellet was transferred into liquid nitrogen immediately. After frozen for 15 min, the cell pellet was stored in -80 until transported to the company.

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