Spontaneously Hypertensive Rats manifest deficits in emotional response to 22-kHz and 50-kHz ultrasonic playback. Olszyński et al.

Published: 2 September 2022| Version 1 | DOI: 10.17632/zyyfbffcc6.1
Robert Filipkowski


Raw data showing rats' responses to ultrasonic playback. Rats, i.e. Spontaneously Hypertensive Rats (SHR) and Wistar rats (used as controls), underwent fear conditioning with 0, 1, 6 or 10 shocks (1 s, 1.0 mA each). Their basic level of vocalization, heart rate and freezing were evaluated before fear conditioning; freezing levels were tested after conditioning (context, context plus cue). Then the rats spent 10 min in silence and were exposed to 10-s-long playbacks of 50-kHz ultrasonic vocalizations (USV), 50-kHz artificial tones, 22-kHz USV, 22-kHz tones in randomized order. Data are organized in 10-s-long intervals. Included are: distance traveled [cm], time spent [%] in the half of the cage next to the speaker, heart rate [HR, bpm], and number of emitted USV in response. The initial 10 min was also analyzed in 2-min-long bins. Data show increase of vocalizations in response to the playbacks, especially 50-kHz ones, increase in HR following 50-kHz playbacks (only in Wistar rats) and decrease in HR after 22-kHz playbacks. Shocked Wistar rats showed higher locomotor activity during 50-kHz playback and a more significant decrease in locomotor activity following 22-kHz playback; they vocalized more often, their USV were longer and at a higher frequency – than in control Wistar rats. However, SHR emitted less USV than Wistar rats, they did not show a 50-kHz playback-induced HR increase, while they showed a profound 22-kHz playback-induced HR decrease. Also, the number of previously delivered conditioning shocks appeared to have no effect on the vocal, locomotor and HR responses of SHR, which were observed in Wistar rats.


Steps to reproduce

Please see M&M section of Olszyński et al. Progress in Neuro-Psychopharmacology & Biological Psychiatry 2022/2023 publication (2022 Aug 22;120:110615).


Polska Akademia Nauk Instytut Medycyny Doswiadczalnej i Klinicznej im M Mossakowskiego


Animal Behavior, Schizophrenia, Attention Deficit Hyperactivity Disorder, Acute Stress, Ultrasonic Vocalization, Heart Rate, Fear Conditioning