Mendeley Data Showcase

Trust can be defined as “a willingness to be vulnerable to another for a given set of tasks” and thus, trust and public health are inextricably linked. State actors are key participants in population health, organizing, among other things, mandates and guidelines that target health behaviors and encourage the uptake of medicines, screenings, diagnostics, and control of health conditions. Effective implementation of these crucial, government-sponsored health efforts is conditional on the public’s belief that the state is trustworthy and has one's best interest in mind – positioning trust in government as a central determinant of public health. Trusting relationships between patients, health systems, and health care providers are also essential, as high-quality, safe care and adherence with healthcare professionals’ recommendations heavily depend upon trust. In many countries, trust in government and health care providers are inseparable, as governments are the primary providers of healthcare. Despite these critical relationships, existing studies that link trust and public health outcomes often focus on contemporaneous factors, many of which are endogenous to public health outcomes (e.g., support for the incumbent political party). For example, Sopory and colleagues reported a comprehensive examination of the phenomenon of trust during public health emergency events among 68 studies from 28 countries that included individuals who were directly affected by a public health emergency. Importantly, no studies from South America or Africa were included. The shortage of research on the sociostructural, historical, economic, and political sources of low trust limits our understanding of how trust deficits might be remedied so as to improve population health. Understanding why trust is low as well as how to repair trust are thus of critical importance.

This dataset contains deidentified information from 33 adolescent girls with (n=21) and without (n=12) type 1 diabetes from 12 to 18 years of age. Parameters in the dataset include general demographics and fracture history, general health information, body composition bone mineral density by DXA, bone microarchitecture and strength by HR-pQCT, blood labs, circulating bone turnover biomarkers, and presence of peripheral neuropathy based on the Michigan Neuropathy Screening Instrument. Full details can be found in the associated files. The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 201908120). Participants provided informed consent for the sharing of de-identified data.

Data collected from 48 adult human participants with unilateral upper extremity peripheral nerve injury. The data include 89 variables for each participant, including measures of performance, hand usage, life-relevant outcomes, and injury and demographic factors. Data for each participant were collected in a single visit, and participants were recruited based on referral from one nerve surgery clinic or one hand therapy clinic in St. Louis MO USA. The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 201701125). Participants provided informed consent for the sharing of de-identified data.

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "Mucosal immunization with ChAd-SARS-CoV-2-S prevents sequential transmission of SARS-CoV-2 to unvaccinated hamsters" published in Science Advances. The data contains viral and antibody titers from hamsters not-vaccinated, mucosally vaccinated, or systemically vaccinated and then exposed to SARS-CoV-2 positive hamsters.

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "Immunogenicity and efficacy of XBB.1.5 rS vaccine against EG.5.1 variant of SARS-CoV-2 in Syrian hamsters," published in Journal of Virology (JVI). The data includes measurements of humoral and cellular immune responses in Syrian hamsters following immunization with the nanoparticle recombinant Spike (S) protein-based COVID-19 vaccine (Novavax, Inc.) from different variants. It also includes data comparing the efficacy of the updated monovalent XBB.1.5 variant vaccine to previous COVID-19 vaccines in inducing XBB.1.5 and EG.5.1 neutralizing antibodies and in protecting against a challenge with the EG.5.1 variant of SARS-CoV-2.

Bourbon virus (BRBV) is an emerging pathogen that can cause severe and fatal disease in humans. BRBV is vectored by Amblyomma americanum (lone star ticks), which are widely distributed across the central, southern, and eastern United States. This dataset describes wild animal species captured in two locations close to St. Louis Metro area and their corresponding BRBV neutralizing activity in serum illustrated by IC50 and IC90 values and their capture sites.

Astroviruses commonly cause of gastrointestinal disease in humans and have been linked to fatal cases of encephalitis. A major barrier to the study of human-infecting astroviruses is the lack of an in vivo model, as previous attempts failed to identify a host that supports viral replication. We describe a novel murine model of infection using astrovirus VA1/HMO-C (VA1), an astrovirus with high seroprevalence in humans. VA1 is cardiotropic and viral RNA levels peak in heart tissue seven days post-inoculation in multiple different murine genetic backgrounds. Infectious VA1 particles could be recovered from heart tissue three- and five-days post-inoculation. Intracellular viral capsid was present in heart tissue by immunostaining and viral RNA was detected in cardiac myocytes, endocardium, and endothelial cells based on fluorescent in situ hybridization and confocal microscopy. Histologically, we identified inflammatory infiltrates consistent with myocarditis in some mice, with viral RNA co-localizing with the infiltrates. These foci contained CD3+ T cells and CD68+ macrophages. Viral RNA levels increased by >10-fold in heart tissue or serum samples from Rag1 or Stat1 knockout mice, demonstrating the role of both adaptive and innate immunity in the response to VA1 infection. Based on the in vivo tropisms, we tested cardiac-derived primary cells and determined that VA1 can replicate in primary human cardiac endothelial cells, suggesting a novel cardiovascular tropism in human cells. This novel in vivo model of a human-infecting astrovirus enables further characterization of the host immune response and reveals a new cardiovascular tropism of astroviruses. Experimental data from astrovirus VA1 infection in mice for the manuscript "Novel murine model of human astrovirus infection reveals a cardiovascular tropism: murine model of astrovirus infection". A total of 199 files containing data including mouse weights, tissue weights, qPCR data, focus forming unit measurement, and imaging files are available.

SARS-CoV-2’s transmission bottleneck remains poorly characterized, in part due to a lack of quantitative measurement tools. To address this, we adapted a SARS-CoV-2 reverse genetics system to generate a pool of >200 isogenic SARS-CoV-2 viruses harboring specific 6-nucleotide barcodes inserted in ORF10, a non-translated ORF. We directly inoculated donor Syrian hamsters intranasally with this barcoded virus pool and exposed a paired naïve contact hamster to each donor. Following exposure, the nasal turbinates, trachea, and lungs were collected, viral titers were measured, and the number of barcodes in each tissue were enumerated to quantify the transmission bottleneck. The duration and route (airborne, direct contact, and fomite) of exposure were varied to assess their impact on the transmission bottleneck. Primarily, the dataset contains (1) fastq files from next-generation sequencing of a small amplicon that contains the barcode of our barcoded viruses and (2) associated count matrices showing how many of each barcode are present in each sample. Secondarily, RT-qPCR results measuring viral titers in various respiratory tissues are also included.

Bourbon virus (BRBV) is an emerging pathogen that can cause severe and fatal disease in humans. BRBV is vectored by Amblyomma americanum (lone star ticks), which are widely distributed across the central, southern, and eastern United States. This data describes human serum neutralizing activity against bourbon virus in a residential cohorts from North Carolina, USA.

Projection-TAGs is a retrograde AAV platform that allows multiplex tagging of projection neurons using RNA barcodes. The dataset contains the sequencing data and analysis meta data, as well as R scripts, that are required to reproduce the snRNA-seq analysis and combinatorial snRNA-seq and snATAC-seq analysis related to Fig 2 (a multi-modal single-cell atlas of mouse cortex), Fig 3 (Projection-TAGs revealed axonal collaterals and complex wiring diagram), Fig 4 (Genomic regulatory networks of cortical cell types using Projection-TAGs), and Fig 5 (Projection-TAGs enable the detection of projection neurons acutely activated by visceral pain) of the manuscript.