BBA Molecular Cell Research

Excel data file containing results for screening of kinase inhibitors against the FGF14:Nav1.6 complex (recombinant proteins stably expressed in HEK293 cells).

BGA - Blood gas results obtained weekly for pRBCs during 8 weeks of storage for N=10. IR results for donor A, B and C - These data include second derivatives spectra of pRBCs samples. The raw spectra were preprocessed by ATR-correction, second derivatve spectra calculation with Savitzky-Golay smoothing and normalization. Biochemical analysis - File with the data from the biochemical analysis - glucose and lactate concentrations. Each measurement was repeated twice for each sample measured weekly during 8 weeks. Fig 5 UVVis raw spectra: is set of a UVVis absorption spectra which represents rate of reoxygenation of pRBCs stored for 1 and 5 weeks treated with subsequent doses of sodium dithionite. In each group N was equal to 3. Fig 2 UVVis raw spectra: comprises a set of UVVis absorption spectra obtained for pRBCs stored for 2, 4, 6 and 8 weeks. In each group N was equal to 3. Fig 3 RS spectra: represents a set of Raman spectra obtained for pRBCs stored for 2, 4, 6 and 8 weeks with 488 nm excitation wavelength. In each group N was equal to 3, each sample was measured in 10 randomly chosen spots, each spectrum was accumulated 10 times. LC-MS/MS data - Detection of intracellular metabolites was performed as defined in manuscript. Briefly, an aliquot of 50 μL of pRBCs was centrifuged (500xg, 10 min, RT, no braking) and divided into two fractions – pure fraction of RBCs (marked as RBC in excel file) and SAGM solution supernatant (marked as KON in excel file).

RNA-Seq data

The upload contains raw data files used for the article "SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization". Article abstract Nucleotide sugar transporters (NSTs) are multitransmembrane proteins, localized in the Golgi apparatus and/or endoplasmic reticulum, which provide glycosylation enzymes with their substrates. It has been demonstrated that NSTs may form complexes with functionally related glycosyltransferases, especially in the N-glycosylation pathway. However, potential interactions of NSTs with enzymes mediating the biosynthesis of mucin-type O-glycans have not been addressed to date. Here we report that UDP-galactose transporter (UGT; SLC35A2) associates with core 1 β-1,3-galactosyltransferase 1 (C1GalT1; T-synthase). This provides the first example of an interaction between an enzyme that acts exclusively in the O-glycosylation pathway and an NST. We also found that SLC35A2 associated with the C1GalT1-specific chaperone Cosmc, and that the endogenous Cosmc was localized in both the endoplasmic reticulum and Golgi apparatus of wild-type HEK293T cells. Furthermore, in SLC35A2-deficient cells protein levels of C1GalT1 and Cosmc were decreased and their Golgi localization was less pronounced. Finally, we identified SLC35A2 as a novel molecular target for the antifungal agent itraconazole. Based on our findings we propose that NSTs may contribute to the stabilization of their interaction partners and help them to achieve target localization in the cell, most likely by facilitating their assembly into larger functional units. The Word document (Data description.docx) contains a description of each file.

A list of human protein uniprot IDs. The proteins were identified by LC-MS/MS in the cellular supernatant of MDA-MB-231 cells, originally published in: F.C. Sigloch, J.D. Knopf, J. Weißer, A. Gomez-Auli, M.L. Biniossek, A. Petrera, et al., Proteomic analysis of silenced cathepsin B expression suggests non-proteolytic cathepsin B functionality, Biochim. Biophys. Acta - Mol. Cell Res. 1863 (2016) 2700–2709. doi:10.1016/j.bbamcr.2016.08.005. https://www.ncbi.nlm.nih.gov/pubmed/27526672