Vaccine

These tables represent the analyzed titles of the news stories and related online comments in Dawn.com in Pakistan.

This video supports the published article Vojtek I, Buchy P, Doherty TM, Hoet B.Would immunization be the same without cross-reactivity? Vaccine. 2019;37(4):539-549. doi: 10.1016/j.vaccine.2018.12.005. In this article, we review the concepts of ‘cross-reactivity’ and ‘cross-protection’ which are both common and important from a public health perspective. Real-world cross-protection data are now available for several vaccines (e.g. those against diseases caused by human papillomavirus and rotavirus). Through this article, we reviewed the growing body of evidence to highlight the full impact of vaccines – beyond vaccine-type disease – that should be taken into consideration when assessing the full value of vaccination programs. With the advent of the next generation of vaccine technologies, we think cross-reactivity might become the solution to creating vaccines against highly antigenically and genetically diverse pathogens.

Vaccine Immune Response, Autoimmunity and Morbidity after Neonatal Blood Exchange Transfusion

The excel shows anti-diphtheria antibody of each ID before and after each dose of reduced diphtheria-tetanus toxoid vaccine and the other excel describe adverse event after each vaccine dose

1) ABC_submitter_instructions.txt- How to use ABCpred_submitter.py/required packages for ABCpred_submitter.py 2) ABCpred_submitter.py- Submits FASTA sequences to ABCpred server. Writes an output file of ABCpred epitopes 3) 117_M_types.txt- The 117 M types in this study. Input for the ABCpred_submitter.py. 4) ABCpred_16mer_epitopes.txt- The 16 residue linear B-cell epitopes for the 117 M types in this study. Output for the ABCpred_submitter.py.

This video supports the two published articles by KP Perrett, et al. Immunogenicity, transplacental transfer of pertussis antibodies and safety following pertussis immunization during pregnancy: Evidence from a randomized, placebo-controlled trial. Vaccine. 2020;38(8):2095-2104, and KP Perrett, et al. Impact of tetanus-diphtheria-acellular pertussis immunization during pregnancy on subsequent infant immunization seroresponses: follow-up from a large randomized placebo-controlled trial. Vaccine. 2020;38(8):2105-2114. These articles describe the results of two clinical trials, respectively. First study - a maternal immunization trial which assessed the safety and immunogenicity of a reduced antigen content diphtheria-tetanus-acellular pertussis (Tdap) vaccination during pregnancy, as well as the transfer of maternal pertussis antibodies through the umbilical cord. Second study - an infant follow-up study which assessed the safety and immunogenicity of diphtheria-tetanus-acellular pertussis-hepatitis B-inactivated poliovirus and Haemophilus influenza type B vaccine (DTPa-HBV-IPV/Hib) when administered to healthy infants born to mothers vaccinated in the maternal immunization trial.

Collection date: 2017 Data type: Household survey results Location: Tanzania Sample size: 517 households across six villages in Tanzania Research goals: Describe household level factors that influence willingness to pay for Newcastle disease vaccines used by smallholder farmers to vaccinate their local chickens. The survey includes a willingness to pay activity (double-bounded contingent valuation).

Dataset use to write this paper

the results of the experiments corresponding to mice with triple negative breast cancer immunized with virus-like particles of parvovirus B19 containing multiple neoepitopes are shown. Female, BALB/c mice, 6–8 weeks of age were used for this study. The mice were kept in micro-isolators, with sterile filter covers, had ad libitum access to water and food, 4T1 mouse mammary carcinoma cells (ATCC, Manassas, Virginia, USA) were maintained for a limited time in vitro by passages in RPMI-1640 medium (Gibco®, Grand Island, New York, USA), containing penicillin (100 U/mL), streptomycin (100 μg/mL) and fungicide (0.75 μg/mL) (Sigma Aldrich, St. Luis, Misuri, USA) and supplemented with 10% FBS (Gibco®) The BALB/c mice were randomly separated into experimental groups (n = 5–8) as follows: vehicle (PBS), WT-VLPs (50μg), WT-VLPs + adjuvant (protoxin Cry1Ac 50 μg), rMe-VLPs (50 μg,) and rMe-VLPs + adjuvant (protoxin Cry1Ac 50 μg). The tumors were induced by subcutaneous injection, into the sixth breast of the mice, of 3 x 103 4T1 cells that were freshly obtained from cell culture, with a viability greater than 90% (evaluated by trypan blue exclusion). The treatments were administered intraperitoneally and peritumorally during the experiment every 7 days, from day 7 post tumor induction. Tumor growth was monitored with a digital caliper every 3 days and tumor volume was calculated according to the formula V = L x S2 / 2, where L is the longest side and S the shortest. On day 36 post-tumor induction, the animals were sacrificed using humane methods. The spleens, inguinal nodes, lungs, and the tumors were obtained for further analysis at indicated days. It is important to mention that in this work, we used a lower number of 4T1 cells (3 x 103) to induce orthotopic tumors in BALB/c mice. This cell concentration was chosen to avoid the induction of necrotic processes in the tumors. In fact, when a higher concentration of cells was used, we observed necrosis. We wanted to avoid the induction of necrosis because it provokes painful tissue lesions in the animals, making it necessary to sacrifice the mice prematurely, and thus complicating the evaluation of the antitumor treatments. Lung metastasis For the analysis of the macro-metastases in the lungs, the same scheme described above was used, resulting in an orthotopic model of metastasis. During sacrifice, the lungs were filled with 1 mL of a 10% India Ink solution (Winsor & Newton, London, UK), in PBS, through the trachea with the help of a cannula. The trachea was blocked by surgical thread and the lungs were extracted and washed 3 times with 10 mL of Fekete´s solution (85 mL 70% ethanol, 10 mL 10% paraformaldehyde, and 5 mL acetic acid) and, the lungs were fixed in the same solution overnight. The macroscopic foci were counted and photographed with a stereoscopic microscope, OLYMPUS SZ CTV (Olympus, Tokyo, Japan).

Raw data for figures in manuscript