Neuroscience

Including the details of TUNEL,WB,MDA,SOD,Cytokines,J-aggregate,Cytochrome C.

Data corresponding to : Laminin-coated Electrodes Improve Cochlear Implant Function and post-Insertion Neuronal Survival Esperanza Bas1, Mir R Anwar1, Stefania Goncalves1, Christine T Dinh1, Olena R Bracho1, Juan A Chiossone1, Thomas R Van De Water 1 Aims: The benefits of Cochlear implant (CI) technology depend among other factors on the proximity of the electrode array to the spiral ganglion neurons. Laminin, a component of the extracellular matrix, regulates Schwann cell proliferation and survival as well as reorganization of actin fibers within their cytoskeleton, which is necessary for myelination of peripheral axons. In this study we explore the effectiveness of laminin-coated electrodes in promoting neuritic outgrowth from auditory neurons towards the electrode array and the ability to reduce acoustic and electric auditory brainstem response (i.e. aABR and eABR) thresholds. Findings: In vitro: Schwann cells and neurites are attracted towards laminin coated surfaces with longer neuritic processes in laminin-coated dishes compared to uncoated dishes. In vivo: Animals implanted with laminin-coated electrodes experience significant decreases in eABR and aABR thresholds at selected frequencies compared to the results from the uncoated electrodes group. One month post implantation there was a greater number of spiral ganglion neurons and neuritic processes projecting into the scala tympani of animals implanted with laminin-coated electrodes compared to animals with uncoated electrodes. Conclusions: These data suggest that Schwann cells are attracted towards laminin-coated electrodes and promote neuritic outgrowth/ guidance and promote the survival of spiral ganglion neurons following electrode insertion trauma.

Raw data from the Inferior Colliculus of 5 big brown bats that were presented with echolocation and communication sounds.

Cochlear synaptic loss indudce by repeated noise exposure in Guinea pigs and mice

Detailed statistical results including F statistics and q value of the data presented in Figures of the paper entitled: Downregulation of aquaporin 9 exacerbates beta-amyloid-induced neurotoxicity in Alzheimer’s disease models in vitro and in vivo.

The experimental design used RNA microarray to compare the brain transcriptome (4mm coronal slice encompassing the nigrostriatal pathway) of Balb/c mice treated with 10 days remote PBM with sham-treated mice. Presented are microarray data, generated using Illumina MouseWG-6 v2.0 Expression BeadChips. The data output is from Illumina GenomeStudio, with Cubic Spline normalization and background correction. "Sample Probe" shows intensities for probes on individual arrays, while "Diff Exp" shows summary data comparing the two experimental groups (using the Illumina custom error model for generating the differential expression p value).

Appendix 1 includes supplementary tables.

Valproic acid (VPA) is widely used in the treatment of epilepsy. However, VPA has been revealed to impair memory in both humans and animals. The adverse effects of VPA are associated with reductions in hippocampal neurogenesis and memory. There are neuroprotective properties exerted by melatonin. As such, the present study investigated the protective effects of melatonin against the reductions of memory and neurogenesis caused by VPA. Male Spraque-Dawley rats received VPA (300 mg/kg) twice a day for 14 days, or melatonin (8 mg/kg/day) for 14 days, or co-treatment with VPA and melatonin for either 14 days (preventive and recovery groups) or 28 days (throughout group). Novel object location (NOL) and novel object recognition (NOR) tests were used to assess spatial memory and non-spatial memory, respectively. Proliferation, survival, and immature neurons in the subgranular zone (SGZ) were examined using Ki-67, BrdU and doublecortin (DCX) immunohistochemistry. Exploration time was scored when animals directed theirs nose toward the objects at a distance less than 2 cm. All sections were quantified at X40 on a Nikon ECLIPSE 80i fluorescence microscope. Ki-67, BrdU and DCX positive cells were considered within the SGZ, which is defined as 3 cell breadths of the internal rim of both blades of the dentate gyrus. The numbers of Ki-67, BrdU, and DCX positive cells in each section were calculated by accumulating cell counts per section for 9 sections per brain and multiplying the results by 15 for Ki67 and 8 for BrdU and DCX. The Student’s t-test and one-way ANOVA were used to analyse data where appropriate. Exploration times from both tests were calculated and converted to a preference index (PI), defined as time spent exploring for novelty in the choice trial as a percentage in comparison to 50% chance. The exploration time of either object placed in the novel location or novel object was significant greater than familiar location or object, demonstrating that the animals had normal cognition. If the PI is significant higher than 50% chance, suggesting that the animals did not have memory deficits. For immunostaining, the number of Ki-67, BrdU and DCX positive cells in control group was compared with valproic acid, melatonin, preventive, recovery, and throughout groups to show changes of neurogenesis.

Description of data files EEG Data files for Fig. 2: • This folder contains data files to generate Fig. 2 Behavioral Data files for Fig. 3: • This folder contains data files to generate Fig. 3 (RMS COP, PL, iTTB). EEG Data files for Figs 4-12: • This folder contains data files to generate Fig. 4 through Fig. 12. • There are 2 folders – one for the TMSSMA group and another for the TMSSHAM group. • Within each subject folder – o .eeg, .vhdr, .vmrk are default files generated by Brain vision software that contains raw data and configurations. o .bvct, .elc are digitized channel files o .txt is some other description for data recording o filenames:  baseline_EO: baseline with eyes open  baseline, 2, 3: baselines with eyes closed  flos: FLOS tasks  trial1, 2, 3: tasks that we performed in order

Spreadsheets include behavioural, immunfluorescence and mulitplex data for all animals in this study.