Neuroscience

Data corresponding to : Laminin-coated Electrodes Improve Cochlear Implant Function and post-Insertion Neuronal Survival Esperanza Bas1, Mir R Anwar1, Stefania Goncalves1, Christine T Dinh1, Olena R Bracho1, Juan A Chiossone1, Thomas R Van De Water 1 Aims: The benefits of Cochlear implant (CI) technology depend among other factors on the proximity of the electrode array to the spiral ganglion neurons. Laminin, a component of the extracellular matrix, regulates Schwann cell proliferation and survival as well as reorganization of actin fibers within their cytoskeleton, which is necessary for myelination of peripheral axons. In this study we explore the effectiveness of laminin-coated electrodes in promoting neuritic outgrowth from auditory neurons towards the electrode array and the ability to reduce acoustic and electric auditory brainstem response (i.e. aABR and eABR) thresholds. Findings: In vitro: Schwann cells and neurites are attracted towards laminin coated surfaces with longer neuritic processes in laminin-coated dishes compared to uncoated dishes. In vivo: Animals implanted with laminin-coated electrodes experience significant decreases in eABR and aABR thresholds at selected frequencies compared to the results from the uncoated electrodes group. One month post implantation there was a greater number of spiral ganglion neurons and neuritic processes projecting into the scala tympani of animals implanted with laminin-coated electrodes compared to animals with uncoated electrodes. Conclusions: These data suggest that Schwann cells are attracted towards laminin-coated electrodes and promote neuritic outgrowth/ guidance and promote the survival of spiral ganglion neurons following electrode insertion trauma.

Including the details of TUNEL,WB,MDA,SOD,Cytokines,J-aggregate,Cytochrome C.

ABF file

Self-reported data and all gait initiation COP and kinematic data.

The experimental design used RNA microarray to compare the brain transcriptome (4mm coronal slice encompassing the nigrostriatal pathway) of Balb/c mice treated with 10 days remote PBM with sham-treated mice. Presented are microarray data, generated using Illumina MouseWG-6 v2.0 Expression BeadChips. The data output is from Illumina GenomeStudio, with Cubic Spline normalization and background correction. "Sample Probe" shows intensities for probes on individual arrays, while "Diff Exp" shows summary data comparing the two experimental groups (using the Illumina custom error model for generating the differential expression p value).

pClamp raw data and Origin files used for Fig. 5

Dataset

pClamp raw data and Origin files used for Fig. 4

These are the .mat files of the tasks which are enlisted in tables 1 and 2 in the paper

pClamp raw data and Origin files used for Fig. 7